Device

Part:BBa_J119394:Design

Designed by: Athena J. Davis   Group: Eckdahl Lab   (2016-06-23)


tClone TetA+SacB: Device for Testing Transciptional Terminators and Riboswitches via GGA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1095
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1241
    Illegal BamHI site found at 3438
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1267
    Illegal NgoMIV site found at 1635
    Illegal NgoMIV site found at 1795
    Illegal AgeI site found at 3385
    Illegal AgeI site found at 3412
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 849
    Illegal BsaI.rc site found at 42


Design Notes

No BsaI sites in SacB coding sequence.


Source

Existing part BBa_J119374 and PCR product of SacB coding sequence.

References